https://doi.org/10.4081/ijwc.2023.103
Cytomorphological analysis of liquid platelet-rich fibrin produced with the DUO fixed angle centrifuge (Process, France) for use in the regenerative therapy of skin ulcers
Submitted: June 3, 2023
Accepted: October 16, 2023
Published: December 14, 2023
Accepted: October 16, 2023
Abstract Views: 159
PDF: 43
Supplementary Materials: 14
PDF (Italiano): 68
Materiali Supplementari (Italiano): 18
Supplementary Materials: 14
PDF (Italiano): 68
Materiali Supplementari (Italiano): 18
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Liquid platelet-rich fibrin (PRF) is an injectable second-generation platelet concentrate rich in platelets, leukocytes and fibrinogen obtained by centrifugation of autologous blood. The aim of this study is to analyze the cellular and Fibrinogen content of various types of liquid PRF (C-PRF liquid, A-PRF liquid, i-PRF, liquid fibrinogen) obtained with the use of DUO Fixed Angle Centrifuge (PRF DUO, Process for PRF, Nice, France) with Vacumed liquid fibrinogen (LF) tubes (code 44909) and/or Original S-PRF Sticky tube. An average accumulation of thrombocytes of almost 1.5 times compared to whole blood was found. Due to the high concentration of platelets, PRF-liquids contain important growth factors for tissue regeneration. In this preliminary study we highlighted that the type of liquid PRF has a higher content of platelets (126.3% vs. 109.5%), monocytes (127.6% vs. 84.6%), with a sufficient content of lymphocytes (192.9% vs. 242.1%) and neutrophil granulocytes (64.6% vs. 64.8%) and fibrinogen (67.9% vs. 87.3%), is the i-PRF (700 rpm×5 min) obtained with Vacumed LF tube (code 44909) with statistically insignificant differences compared to whole blood, while the content of Lymphocytes and Fibrinogen present in i-PRF (700 rpm×5’) obtained with PRF-S-Sticky tube is higher. In comparison, the content of cells and fibrinogen obtained with the two methods of preparing platelet-rich plasma is much lower. This indicates that the liquid PRF is most suitable to be used in various cases of tissue regeneration such as facial aesthetics, intra-articular injection, periulcerative injection, etc. is the i-PRF (700 rpm×5 min) obtained with a Vacumed LF tube.
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How to Cite
Crisci, M., Lepore, G., Feleppa, F., Crisci, A., & Flagiello, F. (2023). Cytomorphological analysis of liquid platelet-rich fibrin produced with the DUO fixed angle centrifuge (Process, France) for use in the regenerative therapy of skin ulcers. Italian Journal of Wound Care, 7(3). https://doi.org/10.4081/ijwc.2023.103
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